human crc cell lines Search Results


96
ATCC str profiling
Str Profiling, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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CLS Cell Lines Service GmbH cell lines hdpscs human dental pulp stem cells 300 702 and hgfs human gingival fibroblasts 300 703
Cell Lines Hdpscs Human Dental Pulp Stem Cells 300 702 And Hgfs Human Gingival Fibroblasts 300 703, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Genecopoeia stable alpha catulin promoter gfp reporter cell lines mda mb 231
Stable Alpha Catulin Promoter Gfp Reporter Cell Lines Mda Mb 231, supplied by Genecopoeia, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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94
ATCC human b lymphoblastoid cell line
Human B Lymphoblastoid Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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93
TaKaRa human hepg2 cell lines
miR-1301 inhibits the migration of <t>HepG2</t> cells in a scratch test. The scratch test was used to investigate the wound healing ability at 0, 12, 24 and 48 h. We observed that the proliferation and migration ability of HepG2 cells in the wounded area was reduced in the miR-1301 group after a 24 and 48 h repair period when compared with the control group. There was no difference between the miR-1301 transfected and the control group at 12 h.
Human Hepg2 Cell Lines, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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95
TaKaRa xy telomerase immortalized human cell line
miR-1301 inhibits the migration of <t>HepG2</t> cells in a scratch test. The scratch test was used to investigate the wound healing ability at 0, 12, 24 and 48 h. We observed that the proliferation and migration ability of HepG2 cells in the wounded area was reduced in the miR-1301 group after a 24 and 48 h repair period when compared with the control group. There was no difference between the miR-1301 transfected and the control group at 12 h.
Xy Telomerase Immortalized Human Cell Line, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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93
Boster Bio neurotrophic factor gdnf elisa kit
A. Light field of SSCs cultured with melatonin and <t>GDNF,</t> bar=50 μm. B. Cell density after being cultured with different cell mediums at 48 h. The initial number was 5*10 4 . C. QRT-PCR and western blot analysis of proliferation, self-renewal and Sertoli cell markers. D. Western blot analysis of proliferation and Sertoli cell markers. *, P<0.05,**, P<0.01.
Neurotrophic Factor Gdnf Elisa Kit, supplied by Boster Bio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Genecopoeia mda mb 468
A. Light field of SSCs cultured with melatonin and <t>GDNF,</t> bar=50 μm. B. Cell density after being cultured with different cell mediums at 48 h. The initial number was 5*10 4 . C. QRT-PCR and western blot analysis of proliferation, self-renewal and Sertoli cell markers. D. Western blot analysis of proliferation and Sertoli cell markers. *, P<0.05,**, P<0.01.
Mda Mb 468, supplied by Genecopoeia, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Genecopoeia t47d
A. Light field of SSCs cultured with melatonin and <t>GDNF,</t> bar=50 μm. B. Cell density after being cultured with different cell mediums at 48 h. The initial number was 5*10 4 . C. QRT-PCR and western blot analysis of proliferation, self-renewal and Sertoli cell markers. D. Western blot analysis of proliferation and Sertoli cell markers. *, P<0.05,**, P<0.01.
T47d, supplied by Genecopoeia, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Genecopoeia hek293 cell line
A. Light field of SSCs cultured with melatonin and <t>GDNF,</t> bar=50 μm. B. Cell density after being cultured with different cell mediums at 48 h. The initial number was 5*10 4 . C. QRT-PCR and western blot analysis of proliferation, self-renewal and Sertoli cell markers. D. Western blot analysis of proliferation and Sertoli cell markers. *, P<0.05,**, P<0.01.
Hek293 Cell Line, supplied by Genecopoeia, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Genecopoeia hela cells
A. Light field of SSCs cultured with melatonin and <t>GDNF,</t> bar=50 μm. B. Cell density after being cultured with different cell mediums at 48 h. The initial number was 5*10 4 . C. QRT-PCR and western blot analysis of proliferation, self-renewal and Sertoli cell markers. D. Western blot analysis of proliferation and Sertoli cell markers. *, P<0.05,**, P<0.01.
Hela Cells, supplied by Genecopoeia, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Genecopoeia scl 76321 g2 a549 cas9 p11 ko cells
A. Light field of SSCs cultured with melatonin and <t>GDNF,</t> bar=50 μm. B. Cell density after being cultured with different cell mediums at 48 h. The initial number was 5*10 4 . C. QRT-PCR and western blot analysis of proliferation, self-renewal and Sertoli cell markers. D. Western blot analysis of proliferation and Sertoli cell markers. *, P<0.05,**, P<0.01.
Scl 76321 G2 A549 Cas9 P11 Ko Cells, supplied by Genecopoeia, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


miR-1301 inhibits the migration of HepG2 cells in a scratch test. The scratch test was used to investigate the wound healing ability at 0, 12, 24 and 48 h. We observed that the proliferation and migration ability of HepG2 cells in the wounded area was reduced in the miR-1301 group after a 24 and 48 h repair period when compared with the control group. There was no difference between the miR-1301 transfected and the control group at 12 h.

Journal: Oncology Reports

Article Title: microRNA-1301-mediated inhibition of tumorigenesis

doi: 10.3892/or.2011.1589

Figure Lengend Snippet: miR-1301 inhibits the migration of HepG2 cells in a scratch test. The scratch test was used to investigate the wound healing ability at 0, 12, 24 and 48 h. We observed that the proliferation and migration ability of HepG2 cells in the wounded area was reduced in the miR-1301 group after a 24 and 48 h repair period when compared with the control group. There was no difference between the miR-1301 transfected and the control group at 12 h.

Article Snippet: Total RNA was extracted from human HepG2 cell lines using TRIzol reagent (Takara Biotechnology Co., Ltd.).

Techniques: Migration, Transfection

miR-1301 inhibits the invasion of HepG2 cells in the Transwell chamber assay. Cell invasion ability was analyzed by the Transwell chamber assay 48 h after miR-1301 transfection. The results showed that the number of migration cells in the miR-1301 group was less than that in control group. This indicated that the invasion ability of HepG2 cells might be inhibited by miR-1301.

Journal: Oncology Reports

Article Title: microRNA-1301-mediated inhibition of tumorigenesis

doi: 10.3892/or.2011.1589

Figure Lengend Snippet: miR-1301 inhibits the invasion of HepG2 cells in the Transwell chamber assay. Cell invasion ability was analyzed by the Transwell chamber assay 48 h after miR-1301 transfection. The results showed that the number of migration cells in the miR-1301 group was less than that in control group. This indicated that the invasion ability of HepG2 cells might be inhibited by miR-1301.

Article Snippet: Total RNA was extracted from human HepG2 cell lines using TRIzol reagent (Takara Biotechnology Co., Ltd.).

Techniques: Transwell Chamber Assay, Transfection, Migration

miR-1301 inhibits cell proliferation. The MTT assay was performed to monitor the proliferation rate of HepG2 cells after miR-1301 transfection. The optical density of each well was measured with a microplate spectrophotometer at 490 nm. The optical density of the miR-1301 group decreased at 24 h (A), 48 h (B), and 72 h (C) after transfection. The proliferation rate of cells decreased with increasing concentrations of inhibitor and increasing transfection time (D).

Journal: Oncology Reports

Article Title: microRNA-1301-mediated inhibition of tumorigenesis

doi: 10.3892/or.2011.1589

Figure Lengend Snippet: miR-1301 inhibits cell proliferation. The MTT assay was performed to monitor the proliferation rate of HepG2 cells after miR-1301 transfection. The optical density of each well was measured with a microplate spectrophotometer at 490 nm. The optical density of the miR-1301 group decreased at 24 h (A), 48 h (B), and 72 h (C) after transfection. The proliferation rate of cells decreased with increasing concentrations of inhibitor and increasing transfection time (D).

Article Snippet: Total RNA was extracted from human HepG2 cell lines using TRIzol reagent (Takara Biotechnology Co., Ltd.).

Techniques: MTT Assay, Transfection, Spectrophotometry

miR-1301 promotes cell apoptosis. Apoptosis of HepG2 cells was observed using fluorescence microscopy through a dual pass filter allowing to visualize the Annexin-V-FITC positive and the propidium iodide positive cells in the same field. The results showed that apoptosis of HepG2 cells increased 48 h after transfection of miR-1301 mimics in the miR-1301 group (Aa) when compared with the control group (Ba). (Aa) Apoptosis cells of the Annexin-V-FITC positive cells in the miR-1301 group; (Ab) cells excited by normal light in the miR-1301 group; (Ba) apoptosis cells of the Annexin-V-FITC positive cells in the control group; (Bb) cells excited by normal light in the control group.

Journal: Oncology Reports

Article Title: microRNA-1301-mediated inhibition of tumorigenesis

doi: 10.3892/or.2011.1589

Figure Lengend Snippet: miR-1301 promotes cell apoptosis. Apoptosis of HepG2 cells was observed using fluorescence microscopy through a dual pass filter allowing to visualize the Annexin-V-FITC positive and the propidium iodide positive cells in the same field. The results showed that apoptosis of HepG2 cells increased 48 h after transfection of miR-1301 mimics in the miR-1301 group (Aa) when compared with the control group (Ba). (Aa) Apoptosis cells of the Annexin-V-FITC positive cells in the miR-1301 group; (Ab) cells excited by normal light in the miR-1301 group; (Ba) apoptosis cells of the Annexin-V-FITC positive cells in the control group; (Bb) cells excited by normal light in the control group.

Article Snippet: Total RNA was extracted from human HepG2 cell lines using TRIzol reagent (Takara Biotechnology Co., Ltd.).

Techniques: Fluorescence, Microscopy, Transfection

A. Light field of SSCs cultured with melatonin and GDNF, bar=50 μm. B. Cell density after being cultured with different cell mediums at 48 h. The initial number was 5*10 4 . C. QRT-PCR and western blot analysis of proliferation, self-renewal and Sertoli cell markers. D. Western blot analysis of proliferation and Sertoli cell markers. *, P<0.05,**, P<0.01.

Journal: Oncotarget

Article Title: Melatonin promotes goat spermatogonia stem cells (SSCs) proliferation by stimulating glial cell line-derived neurotrophic factor (GDNF) production in Sertoli cells

doi: 10.18632/oncotarget.12720

Figure Lengend Snippet: A. Light field of SSCs cultured with melatonin and GDNF, bar=50 μm. B. Cell density after being cultured with different cell mediums at 48 h. The initial number was 5*10 4 . C. QRT-PCR and western blot analysis of proliferation, self-renewal and Sertoli cell markers. D. Western blot analysis of proliferation and Sertoli cell markers. *, P<0.05,**, P<0.01.

Article Snippet: GDNF levels were determined by using a Human glial cell line-derived neurotrophic factor (GDNF) ELISA Kit (BOSTER).

Techniques: Cell Culture, Quantitative RT-PCR, Western Blot

A. ELISA analysis of GDNF levels in the SSCs medium. B. Western Blot analysis of phosphorylation levels of AKT and ERK. *, P<0.05,**, P<0.01.

Journal: Oncotarget

Article Title: Melatonin promotes goat spermatogonia stem cells (SSCs) proliferation by stimulating glial cell line-derived neurotrophic factor (GDNF) production in Sertoli cells

doi: 10.18632/oncotarget.12720

Figure Lengend Snippet: A. ELISA analysis of GDNF levels in the SSCs medium. B. Western Blot analysis of phosphorylation levels of AKT and ERK. *, P<0.05,**, P<0.01.

Article Snippet: GDNF levels were determined by using a Human glial cell line-derived neurotrophic factor (GDNF) ELISA Kit (BOSTER).

Techniques: Enzyme-linked Immunosorbent Assay, Western Blot, Phospho-proteomics